| Catalog No. | HB102106 |
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| Species reactivity | Human |
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| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
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| Host species | Chimeric |
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| Isotype | IgG1 |
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| Clone ID | huDMB5F3 |
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| Biological activity |
huDMB5F3 (0.4-7 nM; 4-min association, 30-min dissociation) binds to recombinant MUC1-X protein with extremely high affinity (K d = 5.89 pM), whereas the monovalent Fab fragment exhibits 100-fold lower affinity due to reduced avidity (K d = 4.84 nM) [1]. huDMB5F3 (62 ng/mL-4 ug/mL; 1-h primary antibody incubation, 45-min secondary antibody incubation) binds specifically to human breast cancer ZR75 cells expressing MUC1, and a binding signal is detectable at concentrations as low as 62 ng/mL (0.4 nM) [1]. huDMB5F3 (incubated at 4°C or 37°C) is efficiently internalized into MUC1-expressing cells in a temperature-dependent manner, localizing to the cell membrane at 4°C and entering the intracellular space at 37°C [1]. huDMB5F3 (0.001-1000 pM; incubated with cells following 5-h seeding) potently and selectively kills MUC1-expressing DA3-TM, T47D, Colo357 and ZR75 cancer cells, with an IC 50 value of approximately 16 pM in Colo357 cells and approximately 3 pM in ZR75 cells; soluble MUC1-Xex abrogates its cytotoxicity [1]. Cell Cytotoxicity Assay [1] Cell Line: DA3-TM (mouse mammary tumor cells expressing human MUC1-TM), T47D human breast cancer cells, Colo357 human pancreatic cancer cells, ZR75 human breast cancer cells Concentration: 0.001, 0.01, 0.1, 1, 10, 100, 1000 pM Incubation Time: incubated with cells following 5-h seeding Result: Showed potent cytotoxicity against MUC1-positive cells: DA3-TM cells were highly sensitive with cytocidal activity at picomolar concentrations. Caused extensive cell killing of T47D cells at picomolar concentrations. Resulted in an IC 50 of approximately 16 pM for Colo357 cells. Resulted in an IC 50 of approximately 3 pM for ZR75 cells. Had cytotoxicity abolished by soluble recombinant MUC1-Xex protein, confirming specificity. Showed no effect on MUC1-negative DA3-PAR cells. |
| Expression system |
Mammalian cells |
| Clonality |
Monoclonal |
| Target |
PEMT, CA 15-3, H23AG, Peanut-reactive urinary mucin, PEM, EMA, Cancer antigen 15-3, MUC-1, Episialin, MUC1-CT, MUC1-beta, Tumor-associated mucin, CD227, Polymorphic epithelial mucin, KL-6, MUC1-alpha, MUC1, PUM, Krebs von den Lungen-6, MUC1-NT, Tumor-associated epithelial membrane antigen, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin |
| Endotoxin level |
Please contact the lab for this information. |
| Purity |
>95% purity as determined by SDS-PAGE. |
| Purification |
Protein A/G purified from cell culture supernatant. |
| Accession |
P15941 |
| Form |
Liquid |
| Storage buffer |
0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
|
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | DMB-5F3, chDMB5F3, huDMB5F3 |
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| Background | Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. |
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| References |
1. Pichinuk E, et al. Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer Res. 2012;72(13):3324-3336. [HB102106] |
| Note |
For research use only. Not suitable for clinical or therapeutic use. |
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