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Recombinant Human CD62P/SELP Protein, C-His (HB885011)

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Recombinant Human CD62P/SELP Protein, C-His

Recombinant Human CD62P/SELP Protein, C-His

Recombinant Human CD62P/SELP Protein, C-His

Recombinant Human CD62P/SELP Protein, C-His

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100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.	HB885011
Description	Recombinant Human CD62P/SELP Protein, C-His (HB885011) expressed in Mammalian Cells, spanning Met1-Ala771. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation.
Expression system	Mammalian Cells
Accession	P16109
Protein length	Met1-Ala771
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>80% as determined by SDS-PAGE.
Predicted molecular weight	85.74 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Leukocyte-endothelial cell adhesion molecule 3, Platelet activation dependent granule-external membrane protein, Granule membrane protein 140, GMP-140, CD62P, SELP, GRMP, PADGEM, CD62 antigen-like family member P, LECAM3, P-selectin, GMRP
Background	P-selectin (CD62P/SELP) is a ~90 kDa protein. Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3). 1. Takada, YK. et al. (2023) Life science alliance 6. PMID: 37184585
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CD62P/SELP Protein, C-His
Bioactivity

Detects Crizanlizumab in indirect ELISAs.

References

HB885011-SDS-PAGE-1.jpg

Datasheet

Recombinant Human CD62P/SELP Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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Recombinant Human CD62P/SELP Protein, C-His