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Recombinant Human ITIH2 Protein, N-His (HB663012)

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Recombinant Human ITIH2 Protein, N-His

Recombinant Human ITIH2 Protein, N-His

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Datasheet

Overview

Catalog No.	HB663012
Description	Recombinant Human ITIH2 Protein, N-His (HB663012) expressed in E. coli, spanning Gly452-Asp702. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P19823
Protein length	Gly452-Asp702
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.12 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SHAP, ITI heavy chain H2, Serum-derived hyaluronan-associated protein, ITI-HC2, Inter-alpha-trypsin inhibitor complex component II, Inter-alpha-inhibitor heavy chain 2, ITIH2, Inter-alpha-trypsin inhibitor heavy chain H2, IGHEP2
Background	Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2) is a ~106 kDa protein. May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ITIH2 Protein, N-His

References

HB663012-SDS-PAGE-1.jpg

Datasheet

Recombinant Human ITIH2 Protein, N-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

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Working Solution

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Recombinant Human ITIH2 Protein, N-His