Home > Products > Recombinant Proteins > Other Proteins

Recombinant Human IFIT2 Protein, N-GST & C-His (HY160012)

Datasheet Share product Copy info

Recombinant Human IFIT2 Protein, N-GST & C-His

Recombinant Human IFIT2 Protein, N-GST & C-His

Price(USD): $

Spec:

50ug
100ug
1mg

Number:

Contact us

Overview
Images
References
Datasheet

Overview

Catalog No.	HY160012
Description	Recombinant Human IFIT2 Protein, N-GST & C-His (HY160012) expressed in E. coli, spanning Met1-Glu472. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P09913
Protein length	Met1-Glu472
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	82.81 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	P54, Interferon-induced 54 kDa protein, Interferon-induced protein with tetratricopeptide repeats 2, CIG-42, G10P2, IFI54, IFIT-2, IFI-54K, ISG-54 K, IFIT2, ISG54
Background	Interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) is a ~54 kDa protein. IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human IFIT2 Protein, N-GST & C-His

References

HY160012-SDS-PAGE-1.jpg

Datasheet

Recombinant Human IFIT2 Protein, N-GST & C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

=

Concentration

×

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

×

V₁ (Stock Vol.)

=

Working Solution

C₂ (Working Conc.)

×

V₂ (Working Vol.)

Recommendation

Anti-Human IFIT2 Polyclonal Antibody

Contact us for custom quotes, bulk requests and any other issues.

Mail: support@abinScience.com

Distributor list

Recombinant Human IFIT2 Protein, N-GST & C-His