ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
26.45 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Blinkin, CT29, Kinetochore-null protein 1, Cancer/testis antigen 29, Bub-linking kinetochore protein, Protein CASC5, Protein D40/AF15q14, ALL1-fused gene from chromosome 15q14 protein, Kinetochore scaffold 1, CASC5, KNL1, Cancer susceptibility candidate gene 5 protein, KIAA1570, AF15q14
Background
Outer kinetochore KNL1 complex subunit KNL1 (CASC5) is a ~265 kDa protein. Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
1. Obuse, C. et al. (2004) Nature cell biology 6, 1135-41. PMID: 15502821
2. Kiyomitsu, T. et al. (2007) Developmental cell 13, 663-676. PMID: 17981135
3. Cheeseman, IM. et al. (2008) Molecular biology of the cell 19, 587-94. PMID: 18045986
4. Pagliuca, C. et al. (2009) PloS one 4, e7640. PMID: 19893618
5. Kiyomitsu, T. et al. (2011) Molecular and cellular biology 31, 998-1011. PMID: 21199919
6. Bolanos-Garcia, VM. et al. (2011) Structure (London, England : 1993) 19, 1691-700. PMID: 22000412
7. Krenn, V. et al. (2012) The Journal of cell biology 196, 451-67. PMID: 22331848
8. Petrovic, A. et al. (2016) Cell 167, 1028-1040.e15. PMID: 27881301
9. Bajaj, R. et al. (2018) Structure (London, England : 1993) 26, 1327-1336.e4. PMID: 30100357
10. Yatskevich, S. et al. (2024) Nature structural & molecular biology 31, 874-883. PMID: 38459127
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human KNL1 Protein, N-His
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