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Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-His (VK466082)

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Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-His

Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-His

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Datasheet

Overview

Catalog No.	VK466082
Description	Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-His (VK466082) expressed in E. coli, spanning Lys138-Val426. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03243
Protein length	Lys138-Val426
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Human adenovirus C (HAdV-5)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	E1B 55 kDa protein, E1B-55K, E1b55K, E1B protein, large T-antigen, E1B-495R
Background	E1B 55 kDa protein is a ~55 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest. 1. Harada, JN. et al. (2002) Journal of virology 76, 9194-206. PMID: 12186903 2. Carson, CT. et al. (2003) The EMBO journal 22, 6610-20. PMID: 14657032 3. Schwartz, RA. et al. (2008) Journal of virology 82, 9043-55. PMID: 18614635 4. Schreiner, S. et al. (2010) Journal of virology 84, 7029-38. PMID: 20484509 5. Pennella, MA. et al. (2010) Journal of virology 84, 12210-25. PMID: 20861261 6. Wimmer, P. et al. (2016) Oncogene 35, 69-82. PMID: 25772236
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-His

References

Datasheet

Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-His.pdf

Formula

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Recombinant HAdV-5 E1B-55K/E1B-495R Protein, N-His