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Recombinant Norovirus p30 Protein, N-His (VK441052)

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Recombinant Norovirus p30 Protein, N-His

Recombinant Norovirus p30 Protein, N-His

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Datasheet

Overview

Catalog No.	VK441052
Description	Recombinant Norovirus p30 Protein, N-His (VK441052) expressed in E. coli, spanning Glu225-Ala519. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q83884
Protein length	Glu225-Ala519
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Norovirus Hu/GI.1
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.80 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Capsid protein VP1, CP, Coat protein, p59, Soluble capsid protein, Protein 30k, p30
Background	Capsid protein VP1 is a ~56 kDa protein. Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulates the genomic RNA and is decorated with VP2 proteins. Attaches virion to target cells by binding histo-blood group antigens (HBGAs) present on gastroduodenal epithelial cells. 1. Prasad, BV. et al. (1999) Science (New York, N.Y.) 286, 287-90. PMID: 10514371 2. Jung, J. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 12828-12832. PMID: 31182604 3. White, LJ. et al. (1997) Journal of virology 71, 8066-72. PMID: 9311906 4. Marionneau, S. et al. (2002) Gastroenterology 122, 1967-77. PMID: 12055602 5. Huang, P. et al. (2003) The Journal of infectious diseases 188, 19-31. PMID: 12825167 6. Tan, M. et al. (2006) Journal of virology 80, 7322-31. PMID: 16840313
Note	For research use only

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Bioactivity

Detected by Nano-7 (Catalog No: VK441063) in indirect ELISA.

References

Datasheet

Recombinant Norovirus p30 Protein, N-His.pdf

Formula

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Recombinant Norovirus p30 Protein, N-His