Recombinant Mouse CD166/ALCAM Protein, C-His (MC678021)

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Overview

Catalog No.	MC678021
Description	Recombinant Mouse CD166/ALCAM Protein, C-His (MC678021) expressed in Mammalian Cells, spanning Trp28-Lys527. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	Q61490
Protein length	Trp28-Lys527
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	59.03 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ALCAM, Activated leukocyte cell adhesion molecule, CD166, CD166 antigen, MEMD
Background	CD166 antigen is a ~65 kDa protein. Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. 1. Chitteti, BR. et al. (2014) Blood 124, 519-29. PMID: 24740813 2. Iolyeva, M. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 978-90. PMID: 23169771 3. Hooker, RA. et al. (2015) Journal of musculoskeletal & neuronal interactions 15, 83-94. PMID: 25730656 4. Weiner, JA. et al. (2004) Molecular and cellular neurosciences 27, 59-69. PMID: 15345243
Note	For research use only

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Datasheet

Recombinant Mouse CD166/ALCAM Protein, C-His.pdf

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Recombinant Mouse CD166/ALCAM Protein, C-His (MC678021)

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