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Recombinant Human DEPTOR Protein, N-GST & C-His (HP016022)

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Overview

Catalog No.	HP016022
Description	Recombinant Human DEPTOR Protein, N-GST & C-His (HP016022) expressed in E. coli, spanning Ser316-Cys409. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q8TB45
Protein length	Ser316-Cys409
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	38.20 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPDC6, DEPTOR, hDEPTOR
Background	DEP domain-containing mTOR-interacting protein is a ~46 kDa protein. Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes. DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy. In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity. 1. Peterson, TR. et al. (2009) Cell 137, 873-86. PMID: 19446321 2. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875 3. Zhao, Y. et al. (2011) Molecular cell 44, 304-16. PMID: 22017876 4. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877 5. Yoon, MS. et al. (2015) Molecular cell 58, 549-56. PMID: 25936805 6. Zhao, L. et al. (2018) The Journal of biological chemistry 293, 4883-4892. PMID: 29382726 7. Wälchli, M. et al. (2021) eLife 10. PMID: 34519268 8. Heimhalt, M. et al. (2021) eLife 10. PMID: 34519269
Note	For research use only

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Datasheet

Recombinant Human DEPTOR Protein, N-GST & C-His.pdf

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Recombinant Human DEPTOR Protein, N-GST & C-His