Recombinant Human ADRA2A Protein, N-His (HY201012)

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Overview

Catalog No.	HY201012
Description	Recombinant Human ADRA2A Protein, N-His (HY201012) expressed in E. coli, spanning Arg233-Phe389. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P08913
Protein length	Arg233-Phe389
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.73 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ADRA2A, ADRA2R, ADRAR, Alpha-2 adrenergic receptor subtype C10, Alpha-2A adrenergic receptor, Alpha-2A adrenoceptor, Alpha-2A adrenoreceptor, Alpha-2AAR
Background	Alpha-2A adrenergic receptor is a ~50 kDa protein. Alpha-2 adrenergic receptors are G protein-coupled receptors for catecholamines that activate the G(i/o) protein pathway, thereby promoting adenylyl cyclase inhibition, ERK1/2 stimulation, and voltage-gated calcium channels suppression. Control a variety of physiological processes, such as regulation of blood pressure, lipolysis and insulin release. ADRA2A and ADRA2C mediates the presynaptic feedback inhibition of neurotransmitter release from noradrenergic nerve terminals in sympathetic and central nervous systems. ADRA2A inhibits transmitter release at high stimulation frequencies, whereas ADRA2C modulates neurotransmission at lower levels of nerve activity. The rank order of potency for agonists of ADRA2A is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. 1. Guyer, CA. et al. (1990) The Journal of biological chemistry 265, 17307-17. PMID: 2170371 2. Li, C. et al. (2012) The Journal of biological chemistry 287, 42784-94. PMID: 23105096 3. Fraser, CM. et al. (1989) The Journal of biological chemistry 264, 11754-61. PMID: 2568356 4. Xu, J. et al. (2022) Science advances 8, eabj5347. PMID: 35245122 5. Garg, A. et al. (2016) JCI insight 1. PMID: 27376152
Note	For research use only

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Recombinant Human ADRA2A Protein, N-His.pdf

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Recombinant Human ADRA2A Protein, N-His (HY201012)

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