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Recombinant Mouse IL4 Protein, N-GST & C-His (MY457022)

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Overview

Catalog No.	MY457022
Description	Recombinant Mouse IL4 Protein, N-GST & C-His (MY457022) expressed in E. coli, spanning His23-Ser140. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P07750
Protein length	His23-Ser140
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	41.49 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	B-cell stimulatory factor 1, BSF-1, Binetrakin, IL-4, IL4, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra
Background	Interleukin-4 is a ~15 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. 1. Lee, F. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 2061-5. PMID: 3083412 2. Sideras, P. et al. (1987) Advances in experimental medicine and biology 213, 227-36. PMID: 3498301 3. Terawaki, S. et al. (2015) The Journal of cell biology 210, 1133-52. PMID: 26416964 4. Moon, ML. et al. (2015) Behavior genetics 45, 451-60. PMID: 25772794 5. Bogale, TMH. et al. (2017) Journal of immunology (Baltimore, Md. : 1950) 198, 2681-2688. PMID: 28202615 6. Edukulla, R. et al. (2015) The Journal of biological chemistry 290, 13510-20. PMID: 25847241 7. Kaplan, MH. et al. (1996) Immunity 4, 313-9. PMID: 8624821
Note	For research use only

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Datasheet

Recombinant Mouse IL4 Protein, N-GST & C-His.pdf

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Recombinant Mouse IL4 Protein, N-GST & C-His