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Recombinant SARS-CoV-2 nsp12 Protein, N-His (VK442052)

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Overview
Catalog No.VK442052
Description
Recombinant SARS-CoV-2 nsp12 Protein, N-His (VK442052) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP0DTD1
Protein lengthArg118-Thr225
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesSevere acute respiratory syndrome coronavirus 2
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names2'-O-methyltransferase nsp16, 3C-like proteinase nsp5, 3CL-PRO, 3CLp, EC:2.1.1.56, EC:2.1.1.57, EC:2.7.7.48, EC:2.7.7.50, EC:3.1.13.-, EC:3.4.19.12, EC:3.4.22.-, EC:3.4.22.69, EC:3.6.4.12, EC:3.6.4.13, EC:4.6.1.-, ExoN, GFL, Growth factor-like peptide, Guanine-N7 methyltransferase nsp14, Hel, Helicase nsp13, Host translation inhibitor nsp1, Leader protein, Main protease, Mpro, NendoU, Non-structural protein 1, Non-structural protein 10, Non-structural protein 12, Non-structural protein 13, Non-structural protein 14, Non-structural protein 15, Non-structural protein 16, Non-structural protein 2, Non-structural protein 3, Non-structural protein 4, Non-structural protein 5, Non-structural protein 6, Non-structural protein 7, Non-structural protein 8, Non-structural protein 9, ORF1ab polyprotein, PL-PRO, PL2-PRO, Papain-like protease nsp3, Papain-like proteinase, Pol, Proofreading exoribonuclease nsp14, RNA-capping enzyme subunit nsp9, RNA-directed RNA polymerase nsp12, RdRp, Replicase polyprotein 1ab, SARS coronavirus main proteinase, Uridylate-specific endoribonuclease nsp15, Viral protein genome-linked nsp9, nsp1, nsp10, nsp12, nsp13, nsp14, nsp15, nsp16, nsp2, nsp3, nsp4, nsp5, nsp6, nsp7, nsp8, nsp9, p65 homolog, pp1ab, rep
Background

Replicase polyprotein 1ab is a ~794 kDa protein. Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein.

Note For research use only
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