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Anti-VSV NP/Nucleoprotein Antibody (1001) (VK434043)

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Anti-VSV NP/Nucleoprotein Antibody (1001)

Anti-VSV NP/Nucleoprotein Antibody (1001)

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Overview

Catalog No.	VK434043
Description	Anti-VSV NP/Nucleoprotein Antibody (1001) [1001] (VK434043) is a human monoclonal antibody detecting Nucleoprotein in FCM, IP. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Applications	FCM, IP
Host species	Human
Isotype	VHH-hFc
Clone ID	1001
Clonality	Monoclonal
Target	NP, Nucleoprotein, Nucleocapsid protein, Protein N
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P03521
RRID	Anti-VSV NP/Nucleoprotein Antibody (1001) (abinScience Cat# VK434043, RRID:AB_3725345)
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Nucleoprotein (NP) is a ~47 kDa protein. Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. The nucleocapsid is bullet-shaped with the tip containing 8 turns of a conical spiral before reaching the helical cylindrical trunk. Nucleocapsid assembly is concomitant with replication, therefore viral replication depends on the intracellular concentration of free N, termed N(0). All replicative products are resistant to nucleases. 1. Green, TJ. et al. (2006) Science (New York, N.Y.) 313, 357-60. PMID: 16778022 2. Ge, P. et al. (2010) Science (New York, N.Y.) 327, 689-93. PMID: 20133572 3. Gumpper, RH. et al. (2018) Journal of virology 92. PMID: 29437970 4. Jenni, S. et al. (2022) Nature communications 13, 4802. PMID: 35970826 5. Zhou, K. et al. (2022) Nature communications 13, 5980. PMID: 36216930 6. Green, TJ. et al. (2011) Journal of virology 85, 2714-22. PMID: 21177817 7. Si, Z. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119, e2111948119. PMID: 35476516
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Anti-VSV NP/Nucleoprotein Antibody (1001)

References

Datasheet

Anti-VSV NP/Nucleoprotein Antibody (1001).pdf

Formula

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Anti-VSV NP/Nucleoprotein Antibody (1001)