Anti-Neisseria meningitidis NadA/Neisseria adhesin A Antibody (F3) (JN971023)

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Datasheet

Overview

Catalog No.

JN971023

Description

Anti-Neisseria meningitidis NadA/Neisseria adhesin A Antibody (F3) [F3] (JN971023) is a human monoclonal antibody detecting Neisseria adhesin A in DB, ELISA, WB.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Neisseria meningitidis serogroup B (strain MC58)

Applications

DB, ELISA, WB

Host species

Human

Isotype

VHH-hFc

Clone ID

Clonality

Monoclonal

Target

nadA, Neisseria adhesin A

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9JXK7

RRID

Anti-Neisseria meningitidis NadA/Neisseria adhesin A Antibody (F3) (abinScience Cat# JN971023, RRID:AB_3727796)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Neisseria adhesin A (NadA) is a ~37 kDa protein. Adheres to and induces bacterial uptake by human epithelial cells (Probable). Upon expression in engineered Y.enterocolitica confers an 11- to 15-fold increase in bacterial adherence and uptake by human epithelial cell lines; part of the uptake is mediated by integrin beta-1 (ITGB1) suggesting it may be a human receptor for NadA. A bacterial cell surface protein; antisera against this protein induce complement-mediated killing of this and other strains.

1. Nägele, V. et al. (2011) The Journal of biological chemistry 286, 20536-46. PMID: 21471204

2. Comanducci, M. et al. (2002) The Journal of experimental medicine 195, 1445-54. PMID: 12045242

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Neisseria meningitidis NadA/Neisseria adhesin A Antibody (F3).
Bioactivity

Detects recombinant NadA (Catalog No: JN971012) in indirect ELISA.

References

Datasheet

Anti-Neisseria meningitidis NadA/Neisseria adhesin A Antibody (F3).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Neisseria meningitidis NadA/Neisseria adhesin A Antibody (F3) (JN971023)

Overview

Images

References

Datasheet

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