Anti-Clostridium botulinum botA/BoNT Antibody (F12) (JN888263)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

JN888263

Description

Anti-Clostridium botulinum botA/BoNT Antibody (F12) [F12] (JN888263) is a mouse monoclonal antibody detecting BoNT/A in ELISA.

Species reactivity

Clostridium botulinum

Applications

ELISA

Host species

Mouse

Isotype

VHH-mFc

Clone ID

F12

Clonality

Monoclonal

Target

Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, botA, bna

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P0DPI0

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Botulinum neurotoxin type A (botA) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302

2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132

3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397

4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517

5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155

6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415

7. Mahrhold, S. et al. (2006) FEBS letters 580, 2011-4. PMID: 16545378

8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274

Note

For research use only

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