Anti-CPV-2 Capsid protein VP2 Polyclonal Antibody (VK761014)

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Overview

Catalog No.

VK761014

Description

Anti-CPV-2 Capsid protein VP2 Polyclonal Antibody (VK761014) is a rabbit polyclonal antibody detecting CPV-2 Capsid protein VP2 in WB, IHC, ELISA.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Canine parvovirustype 2 (CPV-2)

Applications

WB, IHC, ELISA

Host species

Rabbit

Isotype

IgG

Species

Canine parvovirustype 2 (isolate Dog/United States/CPV-d/1988) (CPV-2)

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant CPV-2 Capsid protein VP2 (Phe188-Tyr727(Asn518Glu)).

Target

VP1

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P17455

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Capsid protein VP1, Coat protein VP1

Background

Capsid protein VP1 is a ~80 kDa protein. Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s).

Note

For research use only.

Images

References

Datasheet

Anti-CPV-2 Capsid protein VP2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CPV-2 Capsid protein VP2 Polyclonal Antibody (VK761014)

Overview

Images

References

Datasheet

SDS

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