Anti-FOXO1 Polyclonal Antibody (HC288014)

Name: Anti-FOXO1 Polyclonal Antibody
Brand: abinScience
SKU: HC288014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC288014

Description

Anti-FOXO1 Polyclonal Antibody (HC288014) is a rabbit polyclonal antibody detecting FOXO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FOXO1 (Ser301-Gly655).

Target

Forkhead box protein O1, FKHR, FOXO1A, Forkhead in rhabdomyosarcoma, Forkhead box protein O1A, FOXO1

Purification

Purified by antigen affinity column.

Accession

Q12778

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Forkhead box protein O1 (FOXO1) is a ~69 kDa protein. Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism.

1. Guo, S. et al. (1999) The Journal of biological chemistry 274, 17184-92. PMID: 10358076

2. Zhang, X. et al. (2002) The Journal of biological chemistry 277, 45276-84. PMID: 12228231

3. Daitoku, H. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 10042-7. PMID: 15220471

4. Perrot, V. et al. (2005) Molecular endocrinology (Baltimore, Md.) 19, 2283-98. PMID: 15890677

5. Yuan, Z. et al. (2008) Science (New York, N.Y.) 319, 1665-8. PMID: 18356527

6. Yuan, Z. et al. (2009) The Journal of biological chemistry 284, 11285-92. PMID: 19221179

7. Zhao, Y. et al. (2010) Nature cell biology 12, 665-75. PMID: 20543840

8. Valis, K. et al. (2011) Cancer research 71, 946-54. PMID: 21245099

10. Li, F. et al. (2009) The Journal of biological chemistry 284, 20090-8. PMID: 19483080

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-FOXO1 polyclonal antibody at 1ug/mL on recombinant FOXO1 (Catalog No. HC288012).

References

Datasheet

Anti-FOXO1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-FOXO1 Polyclonal Antibody (HC288014)

Overview

Images

References

Datasheet

SDS

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