Anti-GDF15 Polyclonal Antibody (HV986014)

Name: Anti-GDF15 Polyclonal Antibody
Brand: abinScience
SKU: HV986014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HV986014

Description

Anti-GDF15 Polyclonal Antibody (HV986014) is a rabbit polyclonal antibody detecting GDF15 in ELISA, IHC, WB. Suitable for Human and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GDF15 (Ala197-Ile308).

Target

PLAB, Placental bone morphogenetic protein, MIC1, NRG-1, PTGFB, NSAID-regulated gene 1 protein, GDF15, NAG-1, MIC-1, Prostate differentiation factor, NSAID-activated gene 1 protein, Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, Placental TGF-beta, GDF-15, PDF

Purification

Purified by antigen affinity column.

Accession

Q99988

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Growth/differentiation factor 15 (GDF15/MIC1) is a ~34 kDa protein. Hormone produced in response to various stresses to confer information about those stresses to the brain, and trigger an aversive response, characterized by nausea, vomiting, and/or loss of appetite. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. Acts by binding to its receptor, GFRAL, activating GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes responses to stressful conditions. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues.

1. Tsai, VW. et al. (2013) PloS one 8, e55174. PMID: 23468844

2. Tsai, VW. et al. (2014) PloS one 9, e100370. PMID: 24971956

3. Mullican, SE. et al. (2017) Nature medicine 23, 1150-1157. PMID: 28846097

4. Emmerson, PJ. et al. (2017) Nature medicine 23, 1215-1219. PMID: 28846098

5. Yang, L. et al. (2017) Nature medicine 23, 1158-1166. PMID: 28846099

6. Hsu, JY. et al. (2017) Nature 550, 255-259. PMID: 28953886

7. Xiong, Y. et al. (2017) Science translational medicine 9. PMID: 29046435

8. Patel, S. et al. (2019) Cell metabolism 29, 707-718.e8. PMID: 30639358

9. Coll, AP. et al. (2020) Nature 578, 444-448. PMID: 31875646

10. Klein, AB. et al. (2021) Nature communications 12, 1041. PMID: 33589633

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GDF15 polyclonal antibody at 1ug/mL on recombinant GDF15 (Catalog No. HV986012).

References

Datasheet

Anti-GDF15 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-GDF15 Polyclonal Antibody (HV986014)

Overview

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References

Datasheet

SDS

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