Anti-Human Integrin beta 1/CD29 Recombinant Antibody (K20 ) (HY248107)

Name: Anti-Human Integrin beta 1/CD29 Recombinant Antibody (K20 )
Brand: abinScience
SKU: HY248107
Price: 163 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HY248107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	K20
Clonality	Monoclonal
Target	MSK12, VLA-4 subunit beta, CD29, Integrin beta-1, ITGB1, FNRB, MDF2, GPIIA, Glycoprotein IIa, Fibronectin receptor subunit beta
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P05556
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Integrin beta-1 (CD29/ITGB1) is a ~88 kDa protein. Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. 1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415 2. Fujita, M. et al. (2014) PloS one 9, e96372. PMID: 24789099 3. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536 4. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877 5. Bax, DV. et al. (2003) The Journal of biological chemistry 278, 34605-16. PMID: 12807887 6. Jovanovic, J. et al. (2007) The Journal of biological chemistry 282, 6743-51. PMID: 17158881 7. Schumacher, S. et al. (2021) Science advances 7. PMID: 33962943 8. Takada, YK. et al. (2017) The Journal of biological chemistry 292, 20067-20075. PMID: 29030430 9. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973 10. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072
Note	For research use only.

Images

Bioactivity

Detects Human CD29/ITGB1 in indirect ELISAs.
Flow-cytometry

Flow-cytometry using anti-human CD29 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD29 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD29 antibody monoclonal antibody (Catalog # HY248107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.