Anti-Human CD81 Recombinant Antibody (5A6), APC (HX855137)

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Catalog No.	HX855137
Description	Anti-Human CD81 Antibody (5A6), APC [5A6] (HX855137) is a mouse monoclonal antibody detecting CD81 in FCM, WB. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM, WB
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	5A6
Conjugation	APC
Clonality	Monoclonal
Target	CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1
Endotoxin level	Please contact with the lab for this information.
Accession	P60033
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	CD81 antigen is a ~25 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells. Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production. In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype. Present in MHC class II compartments, may also play a role in antigen presentation. 1. Shoham, T. et al. (2006) Molecular and cellular biology 26, 1373-85. PMID: 16449649 2. van Zelm, MC. et al. (2010) The Journal of clinical investigation 120, 1265-74. PMID: 20237408 3. Zimmerman, B. et al. (2016) Cell 167, 1041-1051.e11. PMID: 27881302 4. Cherukuri, A. et al. (2004) The Journal of biological chemistry 279, 31973-82. PMID: 15161911 5. Sagi, Y. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 1613-8. PMID: 22307619 6. Rocha-Perugini, V. et al. (2013) Molecular and cellular biology 33, 3644-58. PMID: 23858057 7. Secrist, H. et al. (1996) European journal of immunology 26, 1435-42. PMID: 8766544 8. Schick, MR. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 4090-7. PMID: 8409388 9. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Anti-Human CD81 Recombinant Antibody (5A6), APC.pdf

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Anti-Human CD81 Recombinant Antibody (5A6), APC (HX855137)

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