Anti-SeV Fusion Protein Polyclonal Antibody (VK604044)

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50ug
100ug

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Overview

Catalog No.

VK604044

Description

Anti-SeV Fusion Protein Polyclonal Antibody (VK604044) is a rabbit polyclonal antibody detecting Fusion glycoprotein F0 in ELISA, IHC, WB. Suitable for Sendai virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Sendai virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Fusion Protein (Gln26-Thr500).

Target

Fusion glycoprotein F0, Protein F, Fusion glycoprotein F2, Fusion glycoprotein F1, F

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O57295, P04855, Q9DUD9, P04856, P12575

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Fusion glycoprotein F0 (Fusion Protein) is a ~61 kDa protein. Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-F polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Sendai virus/SeV F/Fusion glycoprotein F0 (Catalog No: VK604012)

References

Datasheet

Anti-SeV Fusion Protein Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SeV Fusion Protein Polyclonal Antibody (VK604044)

Overview

Images

References

Datasheet

SDS

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