ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mycobacterium tuberculosis
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
6 kDa early secretory antigenic target, ESAT-6, esxA, ESAT-6-like protein EsxB, 10 kDa culture filtrate antigen CFP-10, CFP-10, Secreted antigenic protein MTSA-10, esxB
Background
6 kDa early secretory antigenic target is a ~9 kDa protein. A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF production but not IL-2; decreases expression of host ATF-2 and JUN transcription factors by affecting T-cell receptors signaling downstream of ZAP70, without cytotoxicity or apoptosis.
1. Renshaw, PS. et al. (2002) The Journal of biological chemistry 277, 21598-603. PMID: 11940590
2. Sørensen, AL. et al. (1995) Infection and immunity 63, 1710-7. PMID: 7729876
3. Stanley, SA. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 13001-6. PMID: 14557536
4. Andersen, P. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 3359-72. PMID: 7897219
5. Wang, X. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3668-77. PMID: 19265145
6. Peng, H. et al. (2011) The Journal of biological chemistry 286, 24508-18. PMID: 21586573
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