Anti-Human RBCK1 Polyclonal Antibody (HP909014)

Name: Anti-Human RBCK1 Polyclonal Antibody
Brand: abinScience
SKU: HP909014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP909014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RBCK1 (Met1-Gly156).

Target

RING finger protein 54, RNF54, RanBP-type and C3HC4-type zinc finger-containing protein 1, C20orf18, XAP4 X-associated protein 4, UBCE7IP3, RBCK1, RING-type E3 ubiquitin transferase HOIL-1, HOIL-1, Heme-oxidized IRP2 ubiquitin ligase 1, XAP3, Ubiquitin-conjugating enzyme 7-interacting protein 3, HBV-associated factor 4

Purification

Purified by antigen affinity column.

Accession

Q9BYM8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

RanBP-type and C3HC4-type zinc finger-containing protein 1 (RBCK1) is a ~57 kDa protein. E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways.

1. Yamanaka, K. et al. (2003) Nature cell biology 5, 336-40. PMID: 12629548

2. Tian, Y. et al. (2007) The Journal of biological chemistry 282, 16776-82. PMID: 17449468

3. Zhang, M. et al. (2008) Cell research 18, 1096-104. PMID: 18711448

4. Kirisako, T. et al. (2006) The EMBO journal 25, 4877-87. PMID: 17006537

5. Tokunaga, F. et al. (2009) Nature cell biology 11, 123-32. PMID: 19136968

6. Gerlach, B. et al. (2011) Nature 471, 591-6. PMID: 21455173

7. Tokunaga, F. et al. (2011) Nature 471, 633-6. PMID: 21455180

8. Ikeda, F. et al. (2011) Nature 471, 637-41. PMID: 21455181

9. Noad, J. et al. (2017) Nature microbiology 2, 17063. PMID: 28481331

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human RBCK1 polyclonal antibody at 1ug/mL on recombinant Human RBCK1 (Catalog No. HP909012).

References

Datasheet

Anti-Human RBCK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RBCK1 Polyclonal Antibody (HP909014)

Overview

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References

Datasheet

SDS

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