ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
19.94 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
RING finger protein 54, RNF54, RanBP-type and C3HC4-type zinc finger-containing protein 1, C20orf18, XAP4, Hepatitis B virus X-associated protein 4, UBCE7IP3, RBCK1, RING-type E3 ubiquitin transferase HOIL-1, HOIL-1, Heme-oxidized IRP2 ubiquitin ligase 1, XAP3, Ubiquitin-conjugating enzyme 7-interacting protein 3, HBV-associated factor 4
Background
RanBP-type and C3HC4-type zinc finger-containing protein 1 (RBCK1) is a ~57 kDa protein. E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways.
1. Yamanaka, K. et al. (2003) Nature cell biology 5, 336-40. PMID: 12629548
2. Tian, Y. et al. (2007) The Journal of biological chemistry 282, 16776-82. PMID: 17449468
3. Zhang, M. et al. (2008) Cell research 18, 1096-104. PMID: 18711448
4. Kirisako, T. et al. (2006) The EMBO journal 25, 4877-87. PMID: 17006537
5. Tokunaga, F. et al. (2009) Nature cell biology 11, 123-32. PMID: 19136968
6. Gerlach, B. et al. (2011) Nature 471, 591-6. PMID: 21455173
7. Tokunaga, F. et al. (2011) Nature 471, 633-6. PMID: 21455180
8. Ikeda, F. et al. (2011) Nature 471, 637-41. PMID: 21455181
9. Noad, J. et al. (2017) Nature microbiology 2, 17063. PMID: 28481331
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human RBCK1 Protein, N-His
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