Anti-ALOX12B Polyclonal Antibody (HF742014)

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Overview

Catalog No.	HF742014
Species reactivity	Human, Mouse, Rat
Applications	ELISA, IHC, WB
Host species	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	E. coli - derived recombinant Human ALOX12B (Leu479-Ile701).
Target	ALOX12B, Epidermis-type lipoxygenase 12, Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase
Purification	Purified by antigen affinity column.
Accession	O75342
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only.

Images

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALOX12B (HF742014) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALOX12B was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet