Anti-E2F1/RBBP3 Polyclonal Antibody (HX082014)

Name: Anti-E2F1/RBBP3 Polyclonal Antibody
Brand: abinScience
SKU: HX082014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX082014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human E2F1/RBBP3 (Val88-Phe437).

Target

Retinoblastoma-binding protein 3, Retinoblastoma-associated protein 1, RBBP-3, Transcription factor E2F1, RBBP3, E2F1, E2F-1, RBAP-1, pRB-binding protein E2F-1, PBR3

Purification

Purified by antigen affinity column.

Accession

Q01094

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Transcription factor E2F1 (RBBP3) is a ~46 kDa protein. Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters.

1. Martínez-Balbás, MA. et al. (2000) The EMBO journal 19, 662-71. PMID: 10675335

2. Stevens, C. et al. (2003) Nature cell biology 5, 401-9. PMID: 12717439

3. García-Alvarez, G. et al. (2007) Biochimica et biophysica acta 1773, 375-82. PMID: 17050006

4. Wang, C. et al. (2007) The Journal of biological chemistry 282, 29902-9. PMID: 17704056

5. Wang, C. et al. (2008) FEBS letters 582, 2793-8. PMID: 18625225

6. Wang, D. et al. (2018) Journal of molecular cell biology 10, 60-73. PMID: 28992046

8. Zaragoza, K. et al. (2010) Molecular and cellular biology 30, 2293-304. PMID: 20176812

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-E2F1/RBBP3 polyclonal antibody at 1ug/mL on recombinant E2F1/RBBP3 (Catalog No. HX082022).

References

Datasheet