Anti-Human POLQ Polyclonal Antibody (HF853014)

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HF853014

Description

Anti-Human POLQ Polyclonal Antibody (HF853014) is a rabbit polyclonal antibody detecting POLQ in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human POLQ (Met1-Met894).

Target

POLH, DNA polymerase eta, DNA polymerase theta, POLQ

Purification

Purified by antigen affinity column.

Accession

O75417

RRID

Anti-Human POLQ Polyclonal Antibody (abinScience Cat# HF853014, RRID:AB_3724098)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA polymerase theta (POLQ) is a ~289 kDa protein. Low-fidelity DNA polymerase with a helicase activity that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery required to repair double-strand breaks in DNA during mitosis. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. MMEJ is required during mitosis to repair persistent double-strand breaks that originate in S-phase. Although error-prone, MMEJ protects against chromosomal instability and tumorigenesis. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs.

1. Seki, M. et al. (2003) Nucleic acids research 31, 6117-26. PMID: 14576298

2. Arana, ME. et al. (2008) Nucleic acids research 36, 3847-56. PMID: 18503084

3. Yoon, JH. et al. (2014) The Journal of biological chemistry 289, 13177-85. PMID: 24648516

4. Ceccaldi, R. et al. (2015) Nature 518, 258-62. PMID: 25642963

5. Kent, T. et al. (2015) Nature structural & molecular biology 22, 230-7. PMID: 25643323

6. Zahn, KE. et al. (2015) Nature structural & molecular biology 22, 304-11. PMID: 25775267

7. Newman, JA. et al. (2015) Structure (London, England : 1993) 23, 2319-2330. PMID: 26636256

8. Kent, T. et al. (2016) eLife 5. PMID: 27311885

9. Kent, T. et al. (2016) Nucleic acids research 44, 9381-9392. PMID: 27591252

10. Wang, Z. et al. (2019) The Journal of biological chemistry 294, 3909-3919. PMID: 30655289

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human POLQ polyclonal antibody at 1ug/mL on recombinant Human POLQ (Catalog No. HF853012).

References

Datasheet