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Recombinant Human POLQ Protein, N-His (HF853021)

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Recombinant Human POLQ Protein, N-His

Recombinant Human POLQ Protein, N-His

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Datasheet

Overview

Catalog No.	HF853021
Description	Recombinant Human POLQ Protein, N-His (HF853021) expressed in Insect Cells, spanning Ser1819-Val2590. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Insect Cells
Accession	O75417
Protein length	Ser1819-Val2590
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	89.00 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	POLH, DNA polymerase eta, DNA polymerase theta, POLQ
Background	DNA polymerase theta (POLQ) is a ~289 kDa protein. Low-fidelity DNA polymerase with a helicase activity that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery required to repair double-strand breaks in DNA during mitosis. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. MMEJ is required during mitosis to repair persistent double-strand breaks that originate in S-phase. Although error-prone, MMEJ protects against chromosomal instability and tumorigenesis. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. 1. Seki, M. et al. (2003) Nucleic acids research 31, 6117-26. PMID: 14576298 2. Arana, ME. et al. (2008) Nucleic acids research 36, 3847-56. PMID: 18503084 3. Yoon, JH. et al. (2014) The Journal of biological chemistry 289, 13177-85. PMID: 24648516 4. Ceccaldi, R. et al. (2015) Nature 518, 258-62. PMID: 25642963 5. Kent, T. et al. (2015) Nature structural & molecular biology 22, 230-7. PMID: 25643323 6. Zahn, KE. et al. (2015) Nature structural & molecular biology 22, 304-11. PMID: 25775267 7. Newman, JA. et al. (2015) Structure (London, England : 1993) 23, 2319-2330. PMID: 26636256 8. Kent, T. et al. (2016) eLife 5. PMID: 27311885 9. Kent, T. et al. (2016) Nucleic acids research 44, 9381-9392. PMID: 27591252 10. Wang, Z. et al. (2019) The Journal of biological chemistry 294, 3909-3919. PMID: 30655289
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human POLQ Protein, N-His

References

HF853021-SDS-PAGE-1.jpg

Datasheet

Recombinant Human POLQ Protein, N-His.pdf

Formula

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Recombinant Human POLQ Protein, N-His