Anti-Human IL4 Antibody (8D4-8) (HY457013)

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1mg

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Overview
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References
Datasheet

Overview

Catalog No.

HY457013

Description

Anti-Human IL4 Antibody (8D4-8) [8D4-8] (HY457013) is a mouse monoclonal antibody detecting IL4 in ELISA, FCM, IHC, IP, Neutralization. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, IHC, IP, Neutralization

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

8D4-8

Clonality

Monoclonal

Target

Binetrakin, B-cell stimulatory factor 1, Pitrakinra, Lymphocyte stimulatory factor 1, IL-4, BSF-1, IL4, Interleukin-4

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P05112

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Interleukin-4 (IL4) is a ~17 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.

1. Carr, C. et al. (1991) Biochemistry 30, 1515-23. PMID: 1993171

2. Yokota, T. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 5894-8. PMID: 3016727

3. Delespesse, G. et al. (1989) Journal of immunology (Baltimore, Md. : 1950) 142, 134-8. PMID: 2521231

4. Hage, T. et al. (1999) Cell 97, 271-81. PMID: 10219247

5. Hülsmeyer, M. et al. (2001) Acta crystallographica. Section D, Biological crystallography 57, 1334-6. PMID: 11526337

6. LaPorte, SL. et al. (2008) Cell 132, 259-72. PMID: 18243101

7. Malabarba, MG. et al. (1995) The Journal of biological chemistry 270, 9630-7. PMID: 7721895

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human IL4 antibody.IL4 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL4 antibody monoclonal antibody (Catalog # HY457013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IL4 Antibody (8D4-8).