Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1) (HX250013)

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1mg

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Overview
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Datasheet

Overview

Catalog No.

HX250013

Description

Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1) [BBM.1] (HX250013) is a mouse monoclonal antibody detecting Beta-2-microglobulin in ELISA, FCM, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, WB

Host species

Mouse

Isotype

IgG2b-kappa

Clone ID

BBM.1

Clonality

Monoclonal

Target

Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P61769

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Note

For research use only.

Images

SEC-HPLC

SEC-HPLC detection for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).
Bioactivity

Detects B2M/Beta-2-microglobulin in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # HX250013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1).
Flow CytoMetry

Flow-cytometry using APC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human B2M antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human B2M monoclonal antibody (Catalog: HX250013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD1a & B2M monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD1a & B2M (Catalog No: HY043011)
Lane 2: negative control