Catalog No. | HV064033 |
---|---|
Species reactivity | Human |
Applications | ELISA, FCM |
Host species | Mouse |
Isotype | IgG2a, kappa |
Clone ID | SAA0343 |
Clonality | Monoclonal |
Target | Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, Ig epsilon chain C region ND, IGHE |
Purification | Protein A/G purified from cell culture supernatant. |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
Note | For research use only. |
Flow-cytometry using anti-human IgE antibody.IgE Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human IgE antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human IgE antibody monoclonal antibody (Catalog # HV064033 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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