Anti-Staphylococcus aureus Alpha-toxin/hly Recombinant Antibody (SAA2205) (JN998063)

Name: Anti-Staphylococcus aureus Alpha-toxin/hly Recombinant Antibody (SAA2205)
Brand: abinScience
SKU: JN998063
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

JN998063

Species reactivity

Staphylococcus aureus

Applications

ELISA

Isotype

scFv-His

Clone ID

SAA2205

Clonality

Monoclonal

Target

Alpha-hemolysin, Alpha-HL, Alpha-toxin, hly, hla

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P09616

RRID

Anti-Staphylococcus aureus Alpha-toxin/hly Antibody (SAA2205) (abinScience Cat# JN998063, RRID:AB_3729625)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

1. Walker, B. et al. (1992) The Journal of biological chemistry 267, 10902-9. PMID: 1587866

2. Wilke, GA. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13473-8. PMID: 20624979

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Staphylococcus aureus Alpha-toxin/hly Antibody (SAA2205)

References

Datasheet

Anti-Staphylococcus aureus Alpha-toxin/hly Recombinant Antibody (SAA2205).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Staphylococcus aureus Alpha-toxin/hly Recombinant Antibody (SAA2205) (JN998063)

Overview

Images

References

Datasheet

SDS

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