Anti-Human IDH1-R132L-specific Antibody (LMab-1) (HF781033)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

HF781033

Description

Anti-Human IDH1-R132L-specific Antibody (LMab-1) [LMab-1] (HF781033) is a mouse monoclonal antibody detecting IDH1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

LMab-1

Clonality

Monoclonal

Target

IDH1, IDH, NADP(+)-specific ICDH, Isocitrate dehydrogenase [NADP] cytoplasmic, PICD, IDP, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O75874

RRID

Anti-Human IDH1-R132L-specific Antibody (LMab-1) (abinScience Cat# HF781033, RRID:AB_3729710)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1-R132L-specific) is a ~46 kDa protein. Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.

1. Geisbrecht, BV. et al. (1999) The Journal of biological chemistry 274, 30527-33. PMID: 10521434

2. Dang, L. et al. (2009) Nature 462, 739-44. PMID: 19935646

Note

For research use only.

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