Anti-IDH1 Polyclonal Antibody (HF781014)

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Datasheet

Overview

Catalog No.

HF781014

Description

Anti-IDH1 Polyclonal Antibody (HF781014) is a rabbit polyclonal antibody detecting IDH1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Microtus mexicanus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Microtus mexicanus, Microtus ochrogaster, Ovis aries, Pongo abelii

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IDH1 (Met1-Leu414).

Target

IDH1, IDH, NADP(+)-specific ICDH, Isocitrate dehydrogenase [NADP] cytoplasmic, PICD, IDP, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase

Purification

Purified by antigen affinity column.

Accession

O75874

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1) is a ~46 kDa protein. Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency.

1. Geisbrecht, BV. et al. (1999) The Journal of biological chemistry 274, 30527-33. PMID: 10521434

2. Dang, L. et al. (2009) Nature 462, 739-44. PMID: 19935646

Note

For research use only.

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