Anti-Fusion Protein Polyclonal Antibody (VK521024)

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Overview
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Datasheet

Overview

Catalog No.

VK521024

Description

Anti-Fusion Protein Polyclonal Antibody (VK521024) is a rabbit polyclonal antibody detecting fusion; F in ELISA, IHC, WB. Suitable for Nipah virus and Hendra virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Nipah virus, Hendra virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant hendra virus Fusion (Ile27-Ser487).

Target

fusion; F

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O89342, Q9IH63

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Fusion glycoprotein F0 is a ~59 kDa protein. Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm.

Note

For research use only

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References

Datasheet