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Anti-Human HIF1A Monoclonal Antibody (1A713) (HS858025)

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Anti-Human HIF1A Monoclonal Antibody (1A713)

Anti-Human HIF1A Monoclonal Antibody (1A713)

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Overview

Catalog No.	HS858025
Description	Anti-Human HIF1A Monoclonal Antibody (1A713) [1A713] (HS858025) is a mouse monoclonal antibody detecting HIF1A in ELISA, IHC, WB. Suitable for Human. Highlights ●Recombinant — Superior batch-to-batch reproducibility with defined sequences. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	ELISA, IHC, WB
Host species	Mouse
Isotype	IgG
Clone ID	1A713
Clonality	Monoclonal
Immunogen	E. coli - derived recombinant human HIF1A (Asp55-Val342).
Target	Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q16665
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Hypoxia-inducible factor 1-alpha (HIF1A) is a ~92 kDa protein. Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. 1. Jaakkola, P. et al. (2001) Science (New York, N.Y.) 292, 468-72. PMID: 11292861 2. Masson, N. et al. (2001) The EMBO journal 20, 5197-206. PMID: 11566883 3. Bae, SH. et al. (2004) Biochemical and biophysical research communications 324, 394-400. PMID: 15465032 4. Choi, SM. et al. (2006) The Journal of biological chemistry 281, 34056-63. PMID: 16973622 5. Berta, MA. et al. (2007) Biochemical and biophysical research communications 360, 646-52. PMID: 17610843 6. Kim, EJ. et al. (2008) Arteriosclerosis, thrombosis, and vascular biology 28, 1796-802. PMID: 18658046 7. Gimm, T. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 4443-58. PMID: 20624928 8. Shan, B. et al. (2012) Endocrine-related cancer 19, 13-27. PMID: 22009797 9. Xu, C. et al. (2018) PLoS pathogens 14, e1007259. PMID: 30125331 10. Bhattacharya, S. et al. (1999) Genes & development 13, 64-75. PMID: 9887100
Note	For research use only.

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Datasheet

Anti-Human HIF1A Monoclonal Antibody (1A713).pdf

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Anti-Human HIF1A Monoclonal Antibody (1A713)