Anti-PEDV Spike Glycoprotein S2 Polyclonal Antibody (VK564074)

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Datasheet

Overview

Catalog No.

VK564074

Description

Anti-PEDV Spike Glycoprotein S2 Polyclonal Antibody (VK564074) is a rabbit polyclonal antibody detecting Spike glycoprotein. Suitable for Porcine epidemic diarrhea virus.

Species reactivity

Porcine epidemic diarrhea virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant PEDV Spike Glycoprotein S2 (Gly777-Val1120).

Target

E2, Peplomer protein, S, S glycoprotein, Spike glycoprotein

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q91AV1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Spike glycoprotein (Spike Glycoprotein S2) is a ~151 kDa protein. S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.

Note

For research use only

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References

Datasheet