

| Catalog No. | ZA998014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Description |
Anti-Renilla reniformis Rluc/Luciferase Polyclonal Antibody (ZA998014) is a rabbit polyclonal antibody detecting Renilla-type luciferase. Suitable for Sea pansy.
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| Species reactivity | Renilla reniformis (Sea pansy) | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Host species | Rabbit | ||||||||
| Isotype | IgG | ||||||||
| Clonality | Polyclonal | ||||||||
| Immunogen | E. coli - derived recombinant Renilla reniformis Rluc/Luciferase (Met1-Gln311). | ||||||||
| Target | Coelenterazine h 2-monooxygenase, EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase | ||||||||
| Endotoxin level | Please contact with the lab for this information. | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Accession | P27652 | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
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| Background | Coelenterazine h 2-monooxygenase (Rluc/Luciferase) is a ~36 kDa protein. Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light. | ||||||||
| Note | For research use only |
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