Renilla reniformis Rluc/Luciferase Recombinant Protein (N-His) (ZA998022)

Name: Renilla reniformis Rluc/Luciferase Recombinant Protein (N-His)
Brand: abinScience
SKU: ZA998022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	ZA998022
Description	Recombinant Renilla reniformis Rluc/Luciferase Protein, N-His (ZA998022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	P27652
Protein length	Met1-Gln311
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Renilla reniformis (Sea pansy)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Coelenterazine h 2-monooxygenase, EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase
Background	Coelenterazine h 2-monooxygenase is a ~36 kDa protein. Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Note	For research use only

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Datasheet

Renilla reniformis Rluc/Luciferase Recombinant Protein (N-His).pdf

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Renilla reniformis Rluc/Luciferase Recombinant Protein (N-His) (ZA998022)

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