Anti-VIP Polyclonal Antibody (HF589014)

Name: Anti-VIP Polyclonal Antibody
Brand: abinScience
SKU: HF589014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF589014

Description

Anti-VIP Polyclonal Antibody (HF589014) is a rabbit polyclonal antibody detecting VIP in ELISA, IHC, WB. Suitable for Human, Bovine, Dog, Capra hircus, and Gadus morhua.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human VIP (Tyr29-Pro165).

Target

Vasoactive intestinal polypeptide, Peptide histidine valine 42, VIP, Peptide histidine methioninamide 27, VIP peptides

Purification

Purified by antigen affinity column.

Accession

P01282

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

VIP peptides is a ~19 kDa protein. VIP is a neuropeptide involved in a diverse array of physiological processes through activating the PACAP subfamily of class B1 G protein-coupled receptors: VIP receptor 1 (VPR1) and VIP receptor 2 (VPR2). Abundantly expressed throughout the CNS and peripheral nervous systems where they primarily exert neuroprotective and immune modulatory roles. Also causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.

1. Kitamura, K. et al. (1992) Biochemical and biophysical research communications 185, 134-41. PMID: 1318039

2. Piper, SJ. et al. (2022) Nature communications 13, 7013. PMID: 36385145

3. Wei, Y. et al. (1996) Journal of neuroendocrinology 8, 811-7. PMID: 8933357

4. Brenneman, DE. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 1159-62. PMID: 3456568

5. Ma, JN. et al. (2004) Biochemical pharmacology 67, 1279-84. PMID: 15013843

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-VIP polyclonal antibody at 1ug/mL on recombinant VIP (Catalog No. HF589012).

References

Datasheet

Anti-VIP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-VIP Polyclonal Antibody (HF589014)

Overview

Images

References

Datasheet

SDS

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