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Human VIP Recombinant Protein (N-GST & C-His) (HF589022)

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Overview
Catalog No.HF589022
Description
Recombinant Human VIP Protein, N-GST & C-His (HF589022) expressed in E. coli, spanning Tyr29-Pro164. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionP01282
Protein lengthTyr29-Pro164
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 43.49 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesIntestinal peptide PHM-27, Intestinal peptide PHV-42, PHM-27, PHV-42, Peptide histidine methioninamide 27, Peptide histidine valine 42, VIP, VIP peptides, Vasoactive intestinal peptide, Vasoactive intestinal polypeptide
Background

VIP peptides is a ~19 kDa protein. VIP is a neuropeptide involved in a diverse array of physiological processes through activating the PACAP subfamily of class B1 G protein-coupled receptors: VIP receptor 1 (VPR1) and VIP receptor 2 (VPR2). Abundantly expressed throughout the CNS and peripheral nervous systems where they primarily exert neuroprotective and immune modulatory roles. Also causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.

1. Kitamura, K. et al. (1992) Biochemical and biophysical research communications 185, 134-41. PMID: 1318039
2. Piper, SJ. et al. (2022) Nature communications 13, 7013. PMID: 36385145
3. Wei, Y. et al. (1996) Journal of neuroendocrinology 8, 811-7. PMID: 8933357
4. Brenneman, DE. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 1159-62. PMID: 3456568
5. Ma, JN. et al. (2004) Biochemical pharmacology 67, 1279-84. PMID: 15013843
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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