Anti-Human MUC16/CA125 Antibody (SAA0160) (HV601207)

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Overview

Catalog No.	HV601207
Description	Anti-Human MUC16/CA125 Antibody (SAA0160) [SAA0160] (HV601207) is a mouse monoclonal antibody detecting CA125 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	SAA0160
Clonality	Monoclonal
Target	Mucin-16, CA-125, Ovarian carcinoma antigen CA125, CA125, MUC-16, Ovarian cancer-related tumor marker CA125, MUC16
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q8WXI7
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Mucin-16 (MUC16/CA-125) is a ~1519 kDa protein. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Note	For research use only.

Images

SEC-HPLC

SEC-HPLC detection for Anti-Human MUC16/CA125 Antibody (ABS1166).
Bioactivity

Detects MUC16/CA125 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human MUC16 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MUC16 antibody monoclonal antibody (Catalog # HV601207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human MUC16 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MUC16 antibody monoclonal antibody (Catalog # HV601207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.