Anti-Human MSLN Antibody (SAA0145) (HC496107)

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Overview

Catalog No.	HC496107
Description	Anti-Human MSLN Antibody (SAA0145) [SAA0145] (HC496107) is a mouse monoclonal antibody detecting MSLN in FCM, IHC. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM, IHC
Host species	Mouse
Isotype	IgG2a, lambda
Clone ID	SAA0145
Clonality	Monoclonal
Target	CAK1 antigen, Mesothelin, MSLN, Pre-pro-megakaryocyte-potentiating factor, MPF
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q13421
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Mesothelin (MSLN) is a ~68 kDa protein. Membrane-anchored forms may play a role in cellular adhesion.
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human MSLN antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MSLN antibody monoclonal antibody (Catalog # HC496107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human MSLN antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MSLN monoclonal antibody (Catalog: HC496107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.