Research Grade Anetumab (HC496016)

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Overview

Catalog No.	HC496016
Description	Anetumab (HC496016) is a research-grade recombinant antibody targeting CAK1 antigen. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-lambda
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	CAK1 antigen, Mesothelin, MSLN, Pre-pro-megakaryocyte-potentiating factor, MPF
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q13421
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	BAY94-9343, unconjugated:BAY86-1903, BAY2287409, AnetumabRavtansine, 1954758-84-9
Background	Mesothelin (MSLN) is a ~68 kDa protein. Membrane-anchored forms may play a role in cellular adhesion.
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Anetumab.
Bioactivity

Detects Human MSLN in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anetumab.
Flow Cytometry

Flow-cytometry using anti-human MSLN antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MSLN monoclonal antibody (Catalog HC496016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.