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Anti-Human TRAC Antibody (T10B9.1A-31), FITC (HF009117)

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Overview

Catalog No.	HF009117
Description	Anti-Human TRAC Antibody (T10B9.1A-31), FITC [T10B9.1A-31] (HF009117) is a mouse monoclonal antibody detecting TRAC in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG2b
Clone ID	T10B9.1A-31
Conjugation	FITC
Clonality	Monoclonal
Target	T cell receptor alpha chain constant, TRAC, TCRA
Endotoxin level	Please contact with the lab for this information.
Accession	P01848
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	T cell receptor alpha chain constant (TRAC) is a ~15 kDa protein. Constant region of T cell receptor (TR) alpha chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. 1. Lefranc, MP. (2014) Frontiers in immunology 5, 22. PMID: 24600447 2. Rossjohn, J. et al. (2015) Annual review of immunology 33, 169-200. PMID: 25493333 3. Brownlie, RJ. et al. (2013) Nature reviews. Immunology 13, 257-69. PMID: 23524462 4. Nikolich-Zugich, J. et al. (2004) Nature reviews. Immunology 4, 123-32. PMID: 15040585
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Human TRAC Antibody (T10B9.1A-31), FITC.pdf

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Anti-Human TRAC Antibody (T10B9.1A-31), FITC