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Anti-Human TCR Vα Antibody (34) (HP760107)

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Anti-Human TCR Vα Antibody (34)

Anti-Human TCR Vα Antibody (34)

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Overview

Catalog No.	HP760107
Description	Anti-Human TCR Vα Antibody (34) [34] (HP760107) is a rat monoclonal antibody detecting TCR variable alpha chain in ELISA, FCM, WB. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	ELISA, FCM, WB
Host species	Rat
Isotype	IgG1, kappa
Clone ID	34
Clonality	Monoclonal
Target	TCR variable alpha chain, T-cell receptor variable alpha chain
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01848
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	T cell receptor alpha chain constant (TCRVα) is a ~15 kDa protein. Constant region of T cell receptor (TR) alpha chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. 1. Lefranc, MP. (2014) Frontiers in immunology 5, 22. PMID: 24600447 2. Rossjohn, J. et al. (2015) Annual review of immunology 33, 169-200. PMID: 25493333 3. Brownlie, RJ. et al. (2013) Nature reviews. Immunology 13, 257-69. PMID: 23524462 4. Nikolich-Zugich, J. et al. (2004) Nature reviews. Immunology 4, 123-32. PMID: 15040585
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Anti-Human TCR Vα Antibody (34)

References

Datasheet

Anti-Human TCR Vα Antibody (34).pdf

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Anti-Human TCR Vα Antibody (34)