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Anti-Human IL18/IL1F4 Antibody (SAA0388), PE (HC517127)

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Overview

Catalog No.	HC517127
Description	Anti-Human IL18/IL1F4 Antibody (SAA0388), PE [SAA0388] (HC517127) is a human monoclonal antibody detecting IL1F4 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	SAA0388
Conjugation	PE
Clonality	Monoclonal
Target	IFN-gamma-inducing factor, IL1F4, Interleukin-1 gamma, IL-18, IL-1 gamma, Interleukin-18, Interferon gamma-inducing factor, IGIF, IL18, Iboctadekin
Endotoxin level	Please contact with the lab for this information.
Accession	Q14116
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Interleukin-18 (IL18/IL1F4) is a ~22 kDa protein. Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. 1. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 10653850 2. Kato, Z. et al. (2003) Nature structural biology 10, 966-71. PMID: 14528293 3. Tsutsumi, N. et al. (2014) Nature communications 5, 5340. PMID: 25500532 4. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714 5. Xia, S. et al. (2021) Nature 593, 607-611. PMID: 33883744
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Human IL18/IL1F4 Antibody (SAA0388), PE.pdf

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Anti-Human IL18/IL1F4 Antibody (SAA0388), PE