Anti-VSV G/Glycoprotein G Antibody (1E12#) (VK422033)

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Datasheet

Overview

Catalog No.

VK422033

Description

Anti-VSV G/Glycoprotein G Antibody (1E12#) [1E12#] (VK422033) is a human monoclonal antibody detecting Glycoprotein G in ELISA, FCM, Neutralization.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Vesicular stomatitis Indiana virus (strain Mudd-Summers) (VSIV)

Applications

ELISA, FCM, Neutralization

Host species

Human

Isotype

IgG1, kappa

Clone ID

1E12#

Clonality

Monoclonal

Target

G, Glycoprotein G

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P0C2X0

RRID

Anti-VSV G/Glycoprotein G Antibody (1E12#) (abinScience Cat# VK422033, RRID:AB_3725348)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Glycoprotein G is a ~57 kDa protein. Attaches the virus to host LDL receptors, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane.

1. Nikolic, J. et al. (2018) Nature communications 9, 1029. PMID: 29531262

2. Roche, S. et al. (2006) Science (New York, N.Y.) 313, 187-91. PMID: 16840692

3. Roche, S. et al. (2007) Science (New York, N.Y.) 315, 843-8. PMID: 17289996

Note

For research use only.

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