Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3) (JN963023)

Datasheet Share product Copy info

Price(USD): $

Spec:

20ug
50ug
100ug
1mg

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

JN963023

Description

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3) [HS3] (JN963023) is a mouse monoclonal antibody detecting Theta-toxin in ELISA, Neutralization, WB.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Clostridium perfringens (strain 13 / Type A)

Applications

ELISA, Neutralization, WB

Host species

Mouse

Isotype

IgG2a

Clone ID

HS3

Clonality

Monoclonal

Target

Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Clostridium perfringens (strain 13 / Type A)

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P0C2E9

RRID

Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3) (abinScience Cat# JN963023, RRID:AB_3729018)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3)
Bioactivity

Detects recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin (Catalog No: JN963012) in indirect ELISA
Western blot

Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: ATG5659 200ng
Lane 2: 1* loading buffer