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Anti-Human Vimentin Reference Antibody (Pritumumab, RUO) (HY550016)

Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)
Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)
Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)
Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)
Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)
Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)
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  • Overview

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  • References

  • Datasheet

  • SDS

Overview
Catalog No.HY550016
Description
Pritumumab (HY550016) is a research-grade recombinant antibody targeting Vimentin. Produced in mammalian cells with native-like glycosylation.
Highlights
  • Research Grade — For PK/PD studies, assay development, and ADA research.
  • Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHuman
IsotypeIgG1-nd
Expression system Mammalian cells
Clonality Monoclonal
Target Vimentin, VIM
Endotoxin level Please contact the lab for this information.
Purity >95% purity as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P08670
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCLNG11, 499212-74-7
Background

Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.

Note For research use only. Not suitable for clinical or therapeutic use.
Images
  • Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)

    SEC-HPLC

    SEC-HPLC detection for Research Grade Pritumumab.

  • Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)

    Bioactivity

    Detects Human VIM/Vimentin in indirect ELISAs.

  • Anti-Human Vimentin Reference Antibody (Pritumumab, RUO)

    SDS-PAGE

    SDS-PAGE for Research Grade Pritumumab

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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